Lab Members


Postdoctoral Associates

Dan Downes, Ph.D.

Ph.D. received from University of Florida in 2017

Phone: 352-294-8398

Research Focus: The focus of my research is the application of NMR and GC/MS based metabolomics towards quantifying intermediates associated with metabolic disease. Furthermore, I am investigating in vivo metabolism of dihydroxyacetone (DHA) through 13C hyperpolarization studies. Together, these complementary modalities provide pre-clinical benchmarks for assessing disruptions of bioenergetic pathways important to metabolic diseases. 
Daniel Downes

Ram Khattri, Ph.D.

Ph.D. received from University of Akron, OH in 2016

Phone: 352-294-8398

Research Focus: My research is focused on nuclear magnetic resonance spectroscopy (NMR) based metabolomics. I work with samples ranging for body fluids to freeze clamped tissues from any living system. Metabolomics and flux analysis are performed based on 1H and 13C NMR spectra.  Quantification of the metabolite levels is accomplished using 1H NMR and multivariate statistical analysis. Metabolic modeling of fluxes using 13C NMR data provides complementary information to the 1H metabolomics, enabling a comprehensive readout of energy metabolism and related pathways.

Rohit Mahar, Ph.D.

Ph.D. received from Jawaharlal Nehru University, New Delhi, India in 2017

Phone: 352-294-8398
Research Focus: My research work is focused on the measuring metabolic flux study in the living systems using the advanced Nuclear Magnetic Resonance (NMR) spectroscopic techiniques. I am using 2H and 1H NMR to measure de novo lipogenesis (DNL) of fatty acids in the liver, where the purtubation in DNL is linked with the non-alchoholic fatty liver disease (NAFLD) and ultimately steatohepatitis. I am also applying standard 1H NMR metabolomics methods to study overall metabolic homeostasis in these models.
  Rohit Mahar

Mukyndan Ragavan, Ph.D.

Ph.D. received from Texas A&M University, College Station in 2014.

Phone: 352-294-8398

Research Focus: My work is focused on developing quantitative methods for assessing metabolic flux in the heart and liver. To this end, I am developing carbon-13 hyperpolarization methodologies targeted on glycolytic metabolism as well as TCA cycle turnover. This includes both technical enhancements as well as the search for new imaging agents. Paired with the hyperpolarization studies, I am applying steady state carbon-13 isotopomer analysis in the same systems. Use of these complementary technologies allows validation of the new hyperpolarization methods, an area of extreme need in the field.


Research Staff

Tatsiana Tsarova
Biological Scientist I

Phone: 352-294-8398



Andrea Allen
Lab Technician

Phone: 352-294-8398

Graduate Students

Alan Carter
Master’s Graduate Student

Phone: 352-294-8398